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姓名 |
朱毅敏 |
性别 |
女 |
职位 |
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职称 |
研究员 |
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电话 |
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传真 |
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电子邮件 |
ymzhu2008@sinano.ac.cn |
邮编 |
215123 |
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地址 |
苏州工业园区若水路398号 |
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简历 |
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朱毅敏,1996年6月获天津医科大学临床医学专业硕士学位;1991年8月-1999年9月,任职天津第一中心医院神经内科,住院医师;1999年9月-2000年6月,天津第一中心医院神经内科,主治医师,总住院医师;2000年7月-2004年7月就读于新加坡国立大学医学院生物化学系,2005 年9 月获博士学位;2003年9月-2005年11月,为新加坡国立大学医学院生物化学系研究员;2005年11月-2008年1月,美国加州大学圣巴巴拉分校化学工程系,博士后研究员。现为我所研究员。主要致力于肿瘤靶向性药物的研究。
目前主要研究领域: 1. 功能性多肽筛选技术; 2. 纳米靶向药物载体构建; 3. 纳米材料生物学效应。
本项目组的主要研究领域包括: 实验室目前的研究重点在于针对肿瘤特异性分子或者特定类型的肿瘤细胞筛选靶向多肽。特异性靶向多肽的筛选主要运用了细菌多肽展示技术以及流式细胞技术。细菌多肽展示是一种蛋白质工程技术,可以在体外研究多肽与肿瘤特异性分子或者特定类型的肿瘤细胞的相互作用。通过基因工程技术,把编码不同的多肽序列的核酸序列转入到细菌中,使多肽连接到细菌表面的骨架蛋白上,形成一个融合蛋白一起表达出来。而流式细胞仪作为一种高通量的检测和分选的仪器,在很短时间里能够完成对目标样品的分析和收集。把流式细胞术和细菌多肽展示技术结合在一起,进行荧光激活细胞分选。把靶细胞或靶分子与表达随机多肽序列的细菌库(表达绿色荧光蛋白)进行孵育,通过流式细胞仪进行检测,并分选出带有荧光的细胞或分子,通过多轮的分选与富集,最后得到能够与靶细胞或靶分子特异性结合的多肽序列。 利用筛选得到的多肽进行更深入的研究与商业化产品的开发,主要分为以下四点:1)基于靶向多肽的微流控芯片检测循环肿瘤细胞。2)肿瘤干细胞特异性结合多肽的筛选及应用。3)蛋白酶底物序列的筛选确定与应用。4) 将纳米技术与多肽相结合,设计、构建具有特异性靶向作用的纳米药物以及此纳米药物的生物效应检测。
近期承担项目: 1.国家自然科学基金 多肽-磁性荧光复合纳米颗粒在肿瘤干细胞检测中的应用基础研究 2012.1-2015.12 58万元
2.国家自然科学基金 多肽功能化的微流芯片 2013.9-2017.12 73万
3.江苏省自然科学基金 载PSGL-1-VE GF纳米粒/rt-PA的复合脂质体治疗深静脉血栓的基础研究 2013.7-2016.6 20万
主要论文: Wang, Anxin; Chen, Lisha; Pu, Kefeng; Zhu, Yimin. (2014) "Identification of stem-like cells in non-small cell lung cancer cells with specific peptides." Cancer letters.
Shigdar, S; Li, Y; Bhattacharya, S; O'Connor, M; Pu, CW; Lin, J; Wang, T; Xiang, DX; Kong, LX; Wei, MQ; Zhu, YM; Zhou, SF; Duan, W. (2014) "Inflammation and cancer stem cells." Cancer letters 345(2): 271-278.
Wang, Anxin; Pu, Kefeng; Dong, Bing; Liu, Yang; Zhang, Liming; Zhang, Zhijun; Duan, Wei; Zhu, Yimin. (2013). "Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells." Journal of Applied Toxicology.
Mingchun, Du; Yimin, Zhu; Lihua, Yuan; Hui, Liang; Chenchen, Mou; Xiaoran, Li; Jie, Sun; Yan, Zhuang; Wen, Zhang; Qin, Shi; Bing, Chen; Jianwu, Dai. (2013). "Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix." Colloids and Surfaces A: Physicochemical and Engineering Aspects 434: 78-87.
Dong, Bing; Wang, Anxin; Yuan, Lihua; Chen, Lisha; Pu, Kefeng; Duan, Wei; Yan, Xiyun; Zhu, Yimin. (2013). "Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis." PloS one 8(1): e54467.
Sarah Shigdar, Jia Lin, Yong Li, Chaoyong James Yang, Mingqian Wei, Yimin Zhu, Hui Liu and Wei Duan (2012). "Cancer stem cell targeting: the next generation of cancer therapy and molecular imaging." Therapeutic delivery 3(2): 227-244.
Rong Pan, Yang Liu, Wei Chen, Graham Dawson, Xin Wang, Yan Li, Bin Dong and Yimin Zhu. (2012). "The toxicity evaluation of nano-trititanate with bactericidal properties in vitro." Nanotoxicology 6(3): 327-337.
Chen, Li-Sha; Wang, An-Xin; Dong, Bing; Pu, Ke-Feng; Yuan, Li-Hua; Zhu, Yi-Min. (2012). "A new prospect in cancer therapy: targeting cancer stem cells to eradicate cancer." Chinese journal of cancer 31(12): 564.
Qingbin Zhang, Xin Wang, Hui Yang, and Yimin Zhu “Multicolor Upconverted Luminescence-Encoded Superparticles via Controlling Self-assembly Based on Hydrophobic Lanthanide-doped NaYF4 Nanocrystals” Journal of Materials Chemistry. 21,12132-12138,2011.
Qingtao Chen, Xin Wang, Fenghua Chen, Qingbin Zhang, Bing Dong, Hui Yang, Guixia Liu and Yimin Zhu. “Functionalization of upconverted luminescent NaYF4 : Yb/Er nanocrystals by folic acid–chitosan conjugates for targeted lung cancer cell imaging”. Journal of Materials Chemistry. 21,7661-7667,2011.
Xuejin Wu, Qingbin Zhang, Xin, Wang, Hui, Yang and Yimin Zhu. “One-pot synthesis of carboxyl-functionalized rare earth fluoride nanocrystals with monodispersity, ultrasmall size and highly-bright luminescence”. European Journal of Inorganic Chemistry,13,2158-2163,2011.
Bing Dong and Yimin Zhu, “Molecular-Targeted Therapy for Cancer” Chinese Journal of Cancer.39(3):370-375,2010. (通讯作者)
Karen Y. Dane, Yimin Zhu, Annalee Nguyen, and Patrick S. Daugherty,“Detection of Caspase-3 Activity using Intracellular Fluorescent Protein FRET” Methods in Molecular Biology series, in press, 2008
Sui Sum Young, Yimin Zhu, Derek Smith, Chandra Verma, Wee Guan Lim, Bee Jen Tan, Jeffrey S. Armstrong, Shufeng Zhou, Senf-Lai Tan, Nam Sang Cheung and Wei Duan, “ The last ten amino acid residues beyond the hydrophobic motif are critical for the catalytic competence and function of protein kinase Cα” Journal of Biological Chemistry, 281 (41): 30768-30781, 2006. Epub Aug 8, 2006.
Wee Guan Lim, Bee Jen Tan, Yimin Zhu, Shufeng Zhou, Jeffrey S. Armstrong, Qiu Tian Li, Qihan Dong, Eli Chan, Derek Smith, Chandra Verma, Seng-Lai Tan and Wei Duan, “The very C-terminus of PRK1/PKN is essential for its activation by RhoA and downstream signaling” Cellular Signaling. 18 (9):1473-81, 2006.
Yimin Zhu, Derek Smith, Chandra Verma, Wee Guan Lim, Bee Jen Tan, Jeffery A Armstrong, Shufeng Zhou, Eli Chan, Seng-Lai Tan, Yi-Zhun Zhu, Nam Sang Cheung and Wei Duan, “The very C-terminus of protein kinase Cε is critical for full catalytic competence but its hydrophobic motif is dispensible for the interaction with 3-phosphoinositide dependent-kinase-1” Cellular Signaling, 18(6): 807-18, 2006.
Yimin Zhu, Wee Guan Lim, Bee Jen Tan, Tian Seng Teo & Wei Duan, “Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells”, Cellular Signalling, 17(9):1125-36, 2005.
Wee Guan Lim, Yimin Zhu, Chern-Hoe Wang, Bee Jen Tan, Jeffrey Armstrong, Terje Dokland , Hongyuan Yang, Yi-Zhun Zhu, Tian Seng Teo and Wei Duan, “The last five amino acid residues at the C-terminus of PRK1/PKN is essential for full lipid responsiveness”, Cellular Signalling, 17(9):1084-97, 2005 (并列第一作者).
Yimin Zhu, Qihan Dong, Bee Jen Tan, Wee Guan Lim, Shufeng Zhou and Wei Duan., “ The PKCα-D294G mutant found in pituitary and thyroid tumors fails to transduce extracellular signals” Cancer Research, 65(11): 4520-4524, 2005.
Yimin Zhu, Donna B. Stolz, Fengli Guo, Mark A. Ross, Simon C. Watkins, Bee Jen Tan, Robert Z. Qi, Ed Manser, Qiu Tian Li, Boon Huat Bay, Tian Seng Teo and Wei Duan, “Signaling via a novel integral plasma membrane pool of a serine/threonine protein kinase PRK1 in mammalian cells”, The FASEB Journal, 18:1722-1724, 2004.
Wei Fan, Jun Ding, Wei Duan and Yimin Zhu, “The influence of magnetic fields exposure on neurite outgrowth in PC 12 rat pheochromocytoma cells”, Journal of Magnetism and Magnetic Materials, 282: 325-328, 2004.
Wei Duan and Yimin Zhu, “Exploring the potential role of PRK1/PKN in pathophysiology of Parkinson’s disease”, Movement Disorders 19, Suppl. 9: S28, 2004
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研究领域 |
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功能性多肽筛选技术 纳米靶向药物载体构建 纳米材料生物学效应
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版权所有2013 中国科学院上海材料与制造大型仪器区域中心
牵头单位:中国科学院上海微系统与信息技术研究所
地址:上海市长宁路865号 | 邮 编:200050 | 电话:021-62511070
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